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human coronavirus oc43  (ATCC)
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ATCC human coronavirus oc43
Cross-reactivity of SARS-N mAbs with human <t>coronavirus</t> antigens. (A) The immunofluorescence assay was performed by using SARS-N mAbs on <t>human</t> <t>coronavirus</t> <t>OC43-infected</t> MRC-5 cells. As a positive control, <t>anti-HCoV</t> was used as the mAb against human coronavirus OC43 N protein, and normal mouse serum was used as the negative control. FM and LM indicate fluorescence and light imagery. None of these mAbs showed cross-reactivity with human coronavirus. (B) Cross-reactivity of SARS-N mAbs was examined by antigen-capture ELISA using human coronavirus OC43 lysates (256 HA unit), BrSARS-N protein (500 ng/well) and PBST buffer with 1% BSA as control.
Human Coronavirus Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cross-reactivity of SARS-N mAbs with human coronavirus antigens. (A) The immunofluorescence assay was performed by using SARS-N mAbs on human coronavirus OC43-infected MRC-5 cells. As a positive control, anti-HCoV was used as the mAb against human coronavirus OC43 N protein, and normal mouse serum was used as the negative control. FM and LM indicate fluorescence and light imagery. None of these mAbs showed cross-reactivity with human coronavirus. (B) Cross-reactivity of SARS-N mAbs was examined by antigen-capture ELISA using human coronavirus OC43 lysates (256 HA unit), BrSARS-N protein (500 ng/well) and PBST buffer with 1% BSA as control.

Journal: Virus Research

Article Title: Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid protein

doi: 10.1016/j.virusres.2006.07.004

Figure Lengend Snippet: Cross-reactivity of SARS-N mAbs with human coronavirus antigens. (A) The immunofluorescence assay was performed by using SARS-N mAbs on human coronavirus OC43-infected MRC-5 cells. As a positive control, anti-HCoV was used as the mAb against human coronavirus OC43 N protein, and normal mouse serum was used as the negative control. FM and LM indicate fluorescence and light imagery. None of these mAbs showed cross-reactivity with human coronavirus. (B) Cross-reactivity of SARS-N mAbs was examined by antigen-capture ELISA using human coronavirus OC43 lysates (256 HA unit), BrSARS-N protein (500 ng/well) and PBST buffer with 1% BSA as control.

Article Snippet: Human coronavirus OC43 (ATCC, VR-1558; HCoV OC43), which was tested to determine cross-reactivity with SARS-N mAb, was prepared from MRC-5 cells.

Techniques: Immunofluorescence, Infection, Positive Control, Negative Control, Fluorescence, Enzyme-linked Immunosorbent Assay, Control